Figure 1.
Pattern recognition of membrane compartments in leaf epidermal tissue at cellular and subcellular resolution. Merged confocal laser microscopic images show Arabidopsis leaf epidermal cells. Top section: GFP-2xFYVE plants were imaged at 40× magnification (scale bar = 20 μm) and images analyzed by the endomembrane script. Bottom section: GFP-PEN1 plants were imaged at 20× magnification (scale bar = 50 μm) and images analyzed by the plasma membrane microdomain script. A and B, Merged pseudo images. C and D, Recognition of epidermal cells is shown by colored lines. E, Recognition of GFP-2xFYVE-labeled endosomal compartments is shown by colored circles. F, Recognition of B. graminis induced GFP-PEN1 accumulation beneath attempted fungal entry sites (indicated by arrowheads) is shown by colored circles. Color coding is random, different colors indicate individual cells and compartments.