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. 2010 Sep 27;154(3):1143–1157. doi: 10.1104/pp.110.161612

Figure 7.

Figure 7.

Growth and biochemical characterization of mmdh1mmdh2 complemented with mMDH. A, Plant growth in short-day growth conditions at 40 d of age in the wild type (Wt), mmdh1mmdh2, and a complemented mmdh1mmdh2 35S:mMDH line. B, Changes in total cellular MDH activity. Bars are means ± se (n = 4). C, Total MDH activity in isolated mitochondria, showing activity in intact mitochondria and activity after organelle rupture with Triton X-100. Bars are means ± se (n = 3). D, Malate-dependent respiratory rate of isolated intact mitochondria at pH 6.5 to maximize NAD-ME activity and at pH 7.5 to maximize MDH activity. Bars are means ± se (n = 3). E, Changes in leaf respiration rate measured as oxygen consumption rate in the dark. Bars are means ± se (n = 5). FW, Fresh weight.