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. 2010 Sep 13;154(3):1158–1171. doi: 10.1104/pp.110.159038

Figure 2.

Figure 2.

Lesion phenotype in aca4/11 can be suppressed by transgene expression of an ACA11-GFP fusion. A, Lesions are shown for 20-d-old leaves from plants cultivated hydroponically. Representative images are shown for knockouts and controls in the Col (top panels) and Ws (bottom panels) ecotypes. B, Photograph of soil-grown plants. Col wild-type (WT), aca4-3/11-5, and aca4-3/11-5 plants transformed by the ACA11-GFP construct under the control of the 35S promoter (35S-ACA11-GFP) or the native promoter (ACA11p-ACA11-GFP) are compared. Rescued lines were recovered at a frequency of 21 out of 28 for the 35S-ACA11-GFP construct (e.g. seed stock 1355–1358) and two out of eight for the ACA11p-ACA11-GFP construct (ss1353 and 1354). C, Immunodetection of the ACA11-GFP fusion. Membrane proteins (30 μg) from wild-type, aca4-3/11-5, and two lines of aca4-3/11-5 plants transformed by the ACA11-GFP construct under the control of the 35S promoter (35S-ACA11-GFP) or the native promoter (ACA11p-ACA11-GFP) were analyzed by immunoblot for their reaction with an antibody raised against GFP. Bands corresponding to the expected size of the GFP-tagged ACA11 were detected at 137 kD.