Figure 8.

Activation of endogenous c-maf gene by overexpression of Pax6. The Pax6 expression plasmid was permanently introduced into C3H10T1/2 cells. Expression of Pax6 and c-maf mRNAs in four independent cell clones (Cl-1–Cl-4, each 20 µg) were determined by RNase protection analysis. RNA from the cell clone transfected only with the neomycin resistance gene and yeast RNA were used as controls (neo and Y, respectively). The same RNA samples (each 5 µg) were probed with a GAPDH probe as controls. Arrows indicate protected bands of c-maf, endogenous Pax6, transfected Pax6 mRNA and the GAPDH probe, respectively. Size markers were used as in Figure 2 (M).