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. 2010 Sep 8;154(3):1272–1280. doi: 10.1104/pp.110.162131

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Figure 2. — Positional cloning and confirmation of MOM1. A, Genetic mapping delimited M-44 to bacterial artificial chromosome clone (BAC) T6D22. B, Structure of MOM1 and position of the M-44/mom1-44 mutation. Boxes, exons; lines, introns. The mom1-44 mutation and T-DNA insertions in mom1-2 and mom1-3 are indicated by arrows. The M-44 mutation is located 400 bp downstream of the MOM1 transcription start site. C, Expression of MOM1 in Col-0, mom1-2, and mom1-3 as shown by RT-PCR. ACTIN was used as a control. D, M-44/mom1-44 is allelic to mom1. M-44/mom1-44 was crossed to wild-type (WT), mom1-2, or mom1-3 plants. LUC imaging was done using 7-d-old F1 wild-type or mom1 mutant seedlings. E, Complementation of mom1-44 as shown by LUC imaging. [See online article for color version of this figure.]