35SP-directed siRNA production in wild-type (WT) and mom1-44 plants is not related to LUC transcriptional read-through. A, Detection of CaMV 35SP-directed siRNA, miR166, and siRNA02 in the indicated genotypes. An ethidium bromide-stained gel corresponding to tRNA and 5S rRNA was used as a loading control. The positions of the size markers are indicated (24 or 21 nucleotides [nt]). B, LUC luminescence in mom1-44, rdr2, and rdr2 mom1-44 mutant plants. The plants shown (from top to bottom) are as follows: wild type, mom1-44, rdr2, and rdr2 mom1-44. C, Expression of LUC in LUC, wild-type, mom1-44, rdr2, and rdr2 mom1-44 plants. Ethidium bromide-stained rRNA was used as a loading control. [See online article for color version of this figure.]