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. 2010 Aug 30;154(3):1335–1346. doi: 10.1104/pp.110.160234

Figure 6.

Figure 6.

Analysis of small RNAs. A, Northern hybridization for seven small RNAs. Small RNA accumulations are greatly reduced or nondetectable in both waf1-1 and waf1-2. Arrowheads indicate larger smearing signals detected only in waf1. Ethidium bromide-stained gels corresponding to 5S rRNA are shown at the bottom. B, Modification of the 3′ end of miR156. Synthesized RNA oligonucleotide (left) does not show increased mobility after incubation without (−β) NaIO4/β-elimination but gains mobility (arrow) after incubation with (+β) NaIO4/β-elimination. nt, RNA without incubation. Wild-type miR156 (center) does not show increased mobility after incubation without (−β) and with (+β) NaIO4/β-elimination. miR156 in waf1-1 (right) gains mobility (arrow) after incubation with NaIO4/β-elimination, indicating that the 3′ end of miR156 in waf1-1 is unmodified. Ethidium bromide-stained gels corresponding to tRNA are shown at the bottom.