Figure 2.

Comparison of reducing sugars in G. manicata seedlings using Benedict’s reagent. A, Top row, homogenates of stems (1 and 2) or a hypocotyl (3) from three different seedlings grown on N-replete (N+) medium. Bottom row, homogenates of stems with no gland (1) or with a single gland (2) or a hypocotyl with three glands (3) from seedlings grown on N-free (N−) medium. B, Estimated concentration of reducing sugars in glands versus stems of three soil-grown, N-stressed seedlings. Stems taken from each plant were divided into segments containing glands (Gland) or those lacking glands (Stem). After completion of the Benedict’s reaction, the A₇₀₀ was measured for each sample. The concentration of reducing sugars in each sample was estimated according to a standard curve constructed using solutions with known concentrations of Glc.