Table I. RT-PCR primers for G. manicata cDNA encoding starch/Suc degradation-related proteins.
F, Forward primer; R, reverse primer.
| Annotation (GenBank No.) | Predicted Size (Region Covered)a | % Positive (Amino Acid)b | Primers for RT-PCR (5′ → 3′) |
| Invertasecell wall (HQ009838) | (103–574 amino acids) | 79 | F:GTCGCTAAGAACCTTGATAGATCAC |
| R:TCTAACCTCTCATAGGTTGGTTCAA | |||
| Invertasecytosolic (HQ009839) | 571 amino acids | 89 | F:GCCTGAATACTACGATGGAAAGCTA |
| R:ATCAACAAGTCCAAGACGCAGA | |||
| Suc synthase (HQ009840) | 811 amino acids | 93 | F:GGCGAGCTCTATCGTTACATAGC |
| R:CACCTGATCAGGGTGATACGGATC | |||
| α-Amylase (HQ009841) | 422 amino acids | 79 | F:GAGTTGTGGCGATTGAAGGATTCG |
| R:GAACAGTGACGGGATTCCAGGAT | |||
| β-Amylase (HQ009842) | 543 amino acids | 76 | F:TGTTGGATGATAACGAGGTAGTGG |
| R:GACTCAAGCTACTTGCAAGCTAAC | |||
| Starch phosphorylase (HQ009843) | (376–818 amino acids) | 80 | F:GGCTGCTGCGGATAATGCAT |
| R:TTCTGATGGGACGAGCAAGAG | |||
| SEX4 (HQ009844) | 381 amino acids | 92 | F:GCTCCCAACAAAGATGGTCATGTC |
| R:GATGTCCATGCGTTAAGAATACTCTC | |||
| Arabinofuranosidase (HQ009845) | 690 amino acids | 82 | F:CACTTCTACAGGATCATCAAAGACTG |
| R:GAAGATATAAGGCTGGAACCTGC | |||
| AGPfasciclin (HQ009846) | (24–404 amino acids) | 77 | F:GCATTCTCCAAAGGCATCGAA |
| R:CTAACGGCTGCGTTCTCGT | |||
| AGPclassical (HQ009847) | 201 amino acids | 91 | F:GCTCGTACCAATCTCGCC |
| R:ACTTTAGGACCTACATACTGTGGC | |||
| TIP41-like protein (HQ009848) | 287 amino acids | 82 | F:ATGGAGAGCCCATTATTCTTCGAG |
| R:CAGACTGATGATGGGAAGCCTC |
a
Coordinates in parentheses refer to those of the protein that best matches the incomplete cDNA sequences from G. manicata.
b
Percentage of positive amino acids compared to the best matched protein obtained through BLASTX.