Figure 5.

Comparison of the temperature dependencies of the isoprene emission rate and the rate constant of the IspS at a constant DMADP pool size based on the dynamic experiments. In these experiments, the DMADP pool size corresponds to the steady-state isoprene emission rate at 33°C (the experiments with simultaneous darkening and change in leaf temperature; see “Materials and Methods”). The rate constant was calculated as the slope of isoprene emission rate versus DMADP pool size during the dark isoprene emission transients (see “Materials and Methods”). Error bars denote sd of four to nine replicate experiments at each temperature. Data fitting as in Figure 2.