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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Cell Physiol. 2010 Nov;225(3):822–828. doi: 10.1002/jcp.22287

Fig. 1.

Fig. 1

A: RT-PCR analysis (upper panel) shows overexpression of TMEM205 in both cisplatin-resistant cell lines (KB-CP20 and 7404-CP20), compared with their parental cell lines (KB-3-1 and BEL-7404). The primers for TMEM205 and GAPDH are described in Materials and Methods. Peptide sequences of TMEM205 were used for generating polyclonal antibodies as highlighted (lower panel). B: an immunoblot showing increased expression of TMEM205 in KB-CP20 and 7404-CP20 cells using an affinity-purified antibody directed to TMEM205, and the lower panel showing the Coomassie blue stained gel as a loading control. C: increased expression of TMEM205 was detected by immunoblotting in human CP-r cell lines KB-CP.5 and KB-CP20, and mouse CP-r cell line, A10, and the lower panel showing a Coomassie blue stained gel as loading control. D: real-time PCR analysis to detect TMEM205 mRNA in normal human tissues, indicating a differential expression pattern with higher levels in tissues with secretory function, such as those of the pancreas, adrenal gland, thyroid, liver, and mammary gland.