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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Cell Physiol. 2010 Nov;225(3):822–828. doi: 10.1002/jcp.22287

Fig. 2.

Fig. 2

A: a predicted TMEM-205 topologic model, which has four transmembrane domains, based on the protein sequence analyzed using the PredictProtein Program (http://www.embl-heidelberg.de/predictprotein/predictprotein.html). B: an immunoblot showing increased expression of TMEM205 in KB-CP20 cells, and much stronger signals in an enhanced membrane fraction (MF) than in whole cell lysates (WCL) as indicated by an arrow. The lower panel shows a Coomassie blue stained gel as a loading control. C & D, KB-CP.3 cells were examined. C: confocal fluorescence images reveal cell surface distribution of TMEM205; D, immuno-electron microscopic images reveal location of TMEM205 in microvilli, C, Cell body; M, Membrane; Mv, Microvilli (the left panel), and an enlarged field is shown in the right panel, where the dark grains (labeled with TMEM205 antibody and colloidal gold) show the localization on the microvilli, indicated by arrows.