GNL3L promotes the G2/M transition via TRF1 stabilization.
Propidium iodide-labeled cell cycle analyses showed that GNL3L knockdown in HeLa cells (siG3L, grey bars) induces a significant increase in the G2/M cell percentage compared to the control knockdown cells (siScr, white bars), and this G2/M arrest phenotype can be reversed by restoring TRF1 expression (siG3L+TRF1, black bars). The siG3L+TRF1 cells show additional S-phase blockage and increased sub-G1 apoptotic cells compared to the siG3L sample. , p < 0.01; , p < 0.001.