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. 2001 Mar 1;29(5):1156–1162. doi: 10.1093/nar/29.5.1156

Figure 3.

Figure 3

Recovery of PMR1 on oligo(dT)–cellulose. Post-nuclear liver extract prepared from 5 g liver from control frogs was fractionated on a discontinuous sucrose step gradient to prepare mRNPs and polysomes (20). These were bound to oligo(dT)–cellulose for 2 h at 4°C and the matrix was washed and eluted with water. The recovered material was dissolved in 20 µl of 10 mM Tris–HCl, pH 8.0, RNA was removed by digestion for 10 min at 37°C with 5 µg of RNase A and the sample was assayed by western blot for PMR1.