Figure 4.

Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either AmpliTaq Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.