Table 1. Optimized parameters for different target DNA sizes.

Target DNA (bp)	260		516		969		1257, 5′ UTR
Analysis mode	Mutation detection	Genotyping	Mutation detection	Genotyping	Mutation detection	Genotyping	Mutation detection	Genotyping
KCl (mM)	85	60	95	75	110	95	122	102
DMSO (%)	5	5	0	0	0	0	0	0
ADP (µM)	100	10	0	0	0	0	0	0
MutL (nM)	180	180	225	225	270	270	288	288

Fixed concentration of target DNA (25 nM), MutH (85 nM), MutS (275 nM), BSA (100 µg/ml), MgCl₂ (6 mM), DTT (1 mM) Tris buffer (20 mM), EDTA (0.4 mM), ATP (1.5 mM) and Expand buffer (0.3×) were used. Mutation detection, mutation detection mode. Genotyping, genotyping mode.