Table 2. Effect of GATC site position on PCR–CRMS efficiency.
| Distance (bp)a |
95 |
64 |
45 |
30 |
15 |
| Relative activity | 1 | 0.5 | 0.13 | BDL | BDL |
aDistance between the GATC site and the end of the PCR product.
BDL, below detection limit.
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