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. 2001 Mar 1;29(5):1087–1096. doi: 10.1093/nar/29.5.1087

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Copyright © 2001 Oxford University Press

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Induction of two different bimolecular G4-DNA by ethidium derivatives. (A) The TR2 oligonucleotide (d-TCAGATAGTTAGGGTTAGGGTTA, 8 µM strand concentration) was incubated at 20°C in a 10 mM Tris/1 mM EDTA buffer containing 100 mM KCl. Ethidium derivatives were added to a final concentration ranging from 0 (left lane) to 15 or 20 µM (right lane). All mixtures were loaded on a non-denaturing 12% polyacrylamide gel (0.5× TBE, 20 mM KCl) and run at 4°C for 6 h (50 V). ssDNA corresponds to the single-stranded species, G4-DNA to the quadruplex. (B) Quantification of the previous gels. Amount (%) of G4-DNA as a function of dye concentration.

Induction of two different bimolecular G4-DNA by ethidium derivatives. (A) The TR2 oligonucleotide (d-TCAGATAGTTAGGGTTAGGGTTA, 8 µM strand concentration) was incubated at 20°C in a 10 mM Tris/1 mM EDTA buffer containing 100 mM KCl. Ethidium derivatives were added to a final concentration ranging from 0 (left lane) to 15 or 20 µM (right lane). All mixtures were loaded on a non-denaturing 12% polyacrylamide gel (0.5× TBE, 20 mM KCl) and run at 4°C for 6 h (50 V). ssDNA corresponds to the single-stranded species, G4-DNA to the quadruplex. (B) Quantification of the previous gels. Amount (%) of G4-DNA as a function of dye concentration.