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. 2001 Mar 1;29(5):1144–1155. doi: 10.1093/nar/29.5.1144

Figure 8.

Figure 8

HA-Dbp8p localizes to the nucleolus. Indirect immunofluorescence was performed with cells expressing HA–Dbp8p from the DBP8 promoter (MCD8-3C [pMCD8-6]). (A) DNA was stained with DAPI. (B) The nucleolar protein Nop1 was detected with polyclonal rabbit anti-Nop1p antibodies, followed by decoration with goat anti-rabbit rhodamine-conjugated antibodies. (C) HA–Dbp8p was detected with monoclonal mouse anti-HA antibody 16B12, followed by decoration with goat anti-mouse fluorescein-conjugated antibodies. (D) Superimposition of the signals obtained from Nop1p and HA–Dbp8p.