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. Author manuscript; available in PMC: 2010 Nov 3.
Published in final edited form as: J Mol Biol. 2007 Jul 3;372(2):298–316. doi: 10.1016/j.jmb.2007.06.079

Figure 1.

Figure 1

Induction of RA target genes in F9 cells as measured by RT-PCR. (A) F9 cells were treated with RA for the indicated times and RNA was harvested. Harvested RNA was assayed by semi-quantitative RT-PCR. Each experiment was repeated at least 3 times; data shown is from one experiment. Linearity of the PCR reaction was demonstrated by serial dilution of the 24 h time point template. Products were visualized by standard gel electrophoresis. (B) Quantitation of RT-PCR products by real time PCR. Results depict the mean of 3 independent experiments (±SE) with each quantitative PCR performed in triplicate. The y-axis of each graph has a different scale.