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. Author manuscript; available in PMC: 2010 Nov 3.
Published in final edited form as: J Mol Biol. 2007 Jul 3;372(2):298–316. doi: 10.1016/j.jmb.2007.06.079

Figure 3.

Figure 3

Recruitment of pCIP and p300 to RAREs in F9 cells during RA treatment. F9 cells were treated with RA for various times. Cells were then fixed with formaldehyde and processed into soluble chromatin. Chromatin samples were immunoprecipitated with (A) anti-pCIP antibody or (B) anti-p300 antibody, and bound DNA was quantitated by real time PCR. For comparison each target locus was graphed with the Hoxb1 −18kb 3' negative control locus. Each experiment was repeated at least three times, and the quantitative PCR analyses were performed in triplicate for each sample. The data are presented as percentages of input DNA before immunoprecipitation (mean ± SE). Statistical analyses were performed with Graphpad Prism 4.0. Samples that were statistically (p < 0.05) different from time 0 samples were denoted with a * symbol.