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. 2001 Mar 1;29(5):1047–1053. doi: 10.1093/nar/29.5.1047

Figure 1.

Figure 1

Transactivation by DR1-binding proteins present in rat liver nuclear extracts. (A) Schematic representation of plasmid templates used in cell-free transcription. Plasmids containing AdMLP linked to G-free cassettes 260 or 380 bp in length were used as control templates. Four copies of DR1 element or two copies of the CRBPII-RXRE were cloned upstream of the AdMLP linked to a G-free cassette of 380 bp length. (B) Cell-free transcription assays were performed (as described in Materials and Methods) with the control templates pAdMLP380 and pAdMLP260 (lane 1), pDR1-AdMLP380 and pAdMLP260 (lanes 2 and 3) or pRXRE-AdMLP380 and pAdMLP260 (lanes 4 and 5). The arrow and asterisk represent transcripts generated from 380 and 260 bp G-free cassettes, respectively. Addition of 9-cis-RA (10–5 M) had no significant effect on transactivation from pDR1-AdMLP380 (lane 3) or pRXRE-AdMLP380 (lane 5).