Figure 5. Interaction between human plasminogen and recombinant α-enolase variants.

Binding was analyzed in a ligand blot experiment (A) with immobilized recombinant α-enolase variants (5 µg and 1 µg) and human plasminogen as soluble analyte. Designation of the original oral streptococcal strain and the IPM are given together with the apparent dissociation constants, which where determined by surface plasmon resonance measurements (B–F) at different analyte concentrations (4 µM, 2 µM and 1 µM α-enolase). Although curve shapes suggested interactions of higher complexity, apparent dissociation constants were determined based on the Langmuir model for 1∶1 interaction.