Figure 4.
Histological analysis of lungs of BALB/c mice vaccinated and challenged with virulent strain of Mycobacterium tuberculosis. Five BALB/c mice per group were immunized by intranasal route with a dose of different formulations containing mRNA-Hsp65 or EF-1α mRNA. The BCG group received 1 dose of BCG Moreau subcutaneously. Thirty days after the last immunization animals were challenged with 105 bacilli of the virulent strain of M. tuberculosis by H37RV intranasal route. Thirty days after challenge, animals were sacrificed and their lungs extracted for the recovery histology. 1-Non vaccinated group, 2-BCG group, 3-EF-1α mRNA 5 ug group, 4-EF-1α mRNA 10 μg group, 5-mRNA-Hsp65 5 ug group, 6-mRNA-Hsp65 10 μg group. Staining was performed with hematoxylin and eosin (HE), increased original image of 100×.