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. 2010 Oct 20;10:77. doi: 10.1186/1472-6750-10-77

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Copyright ©2010 Lorenzi et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunostimulatory activity of mRNA-Hsp65. Five BALB/c mice per group mice were immunized with one dose of mRNA-Hsp65 (10 μg per mouse in 100 μL volume). Not immunized group received only the Ringer's solution. Two weeks after the immunization splenic cells are isolated and stimulated with 20 μg/ml of Hsp65 protein, 48 hours after stimulation the production of (A) IFN-gamma and (B) TNF-alpha by were determined by ELISA. *p < 0.05 were considered significant when compared to not immunized group.