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HIPK2 activates ATF1 Transcription function. GAL4-DBD-luciferase plasmid was transfected into HEK293 cells together with GAL4 or GAL4-ATF1 (1–228) plus HIPK2 or PKA expression plasmid. The luciferase assay was carried out 48 hours after transfection. GAL4-ATF1 plus empty-vector-transfected sample was set as 1.0. DNA transfection was carried out in duplicate in each experiment and the result of four independent experiments and standard errors are shown.
