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. 2010 Oct 27;123(22):3863–3871. doi: 10.1242/jcs.073627

Fig. 8.

Fig. 8.

HIPK2 deficiency affects expression of ferritin H and other ARE-regulated genes in genotoxic stress. (A) HIPK2+/+ or HIPK2−/− MEF cells were treated with 0.5 or 2.0 μg/ml doxorubicin (Dox), 10 or 50 μM etoposide (Eto) or 10 μM sodium arsenite (As). Twenty hours after the treatment, total RNA was isolated and subjected to real-time PCR for expression of ferritin H and L. (B) HIPK2+/+ or HIPK2−/− MEF cells were treated with 50 μM etoposide for 20 hours and expression of ferritin H (FH), ferritin L (FL), NAD(P)H quinone oxidoreductase-1 (NQO1), glutathione S-transferase-α (GSTa), or heme oxygenase-1 (HO1) mRNA was measured by real-time PCR. (C) SH-SY5Y cells were transfected with non-targeting (siControl) or HIPK2-targeting (siHIPK2) siRNA and incubated for 24 hours. Cells were then treated with 2 μM or 10 μM Etoposide for 48 hours and ferritin H and NQO1 mRNA expression was measured by real-time PCR. In B and C, mean and standard errors from three independent experiments were shown. *P<0.05, as determined by a Student's t-test.