Table 3.
Stability of candidate reference genes in sexual difference of the same tissues of Bactrocera dorsalis evaluated by Normfinder.
| Ranking order | Gene name | Stability value in midgut | Gene name | Stability value in Malpighian tubules | Gene name | Stability value in fat body |
|---|---|---|---|---|---|---|
| 1 |
ACT5 |
0.050 |
ACT3 |
0.180 |
EF1α |
0.210 |
| 2 |
α-TUB |
0.136 |
ACT2 |
0.200 |
ACT5 |
0.215 |
| 3 |
ACT2 |
0.170 |
α-TUB |
0.240 |
ACT1 |
0.325 |
| 4 |
ACT3 |
0.277 |
EF1α |
0.364 |
α-TUB |
0.361 |
| 5 |
EF1α |
0.295 |
β-TUB |
0.463 |
G6PDH |
0.398 |
| 6 |
18S |
0.385 |
GAPDH |
0.478 |
ACT2 |
0.440 |
| 7 |
GAPDH |
0.431 |
ACT5 |
0.498 |
18S |
0.494 |
| 8 |
β-TUB |
0.458 |
ACT1 |
0.542 |
GAPDH |
0.512 |
| 9 |
G6PDH |
0.601 |
G6PDH |
0.657 |
ACT3 |
0.641 |
| 10 | ACT1 | 0.657 | 18S | 0.816 | β-TUB | 0.755 |