Table 1. Sequences of the oligonucleotides.
Positions |
Sequence |
Strand |
–5 | 5′-ctcttagtca ggaaYatgtc tctatgctgg gagcaaaggc-3′ | (1) |
3′-gagaatcagt ccttNtacaX agatacgacc ctcgtttccg-5′ | (2) | |
–3 | 5′-ctcttagtca ggaataYgtc tctatgctgg gagcaaaggc-3′ | |
3′-gagaatcagt ccttatNcaX agatacgacc ctcgtttccg-5′ | ||
–1 | 5′-ctcttagtca ggaatatgYc tctatgctgg gagcaaaggc-3′ | |
3′-gagaatcagt ccttatacNX agatacgacc ctcgtttccg-5′ | ||
+1 | 5′-ctcttagtca ggaatatgtc Yctatgctgg gagcaaaggc-3′ | |
3′-gagaatcagt ccttatacaX Ngatacgacc ctcgtttccg-5′ | ||
+3 | 5′-ctcttagtca ggaatatgtc tcYatgctgg gagcaaaggc-3′ | |
3′-gagaatcagt ccttatacaX agNtacgacc ctcgtttccg-5′ | ||
+5 | 5′-ctcttagtca ggaatatgtc tctaYgctgg gagcaaaggc-3′ | |
3′-gagaatcagt ccttatacaX agatNcgacc ctcgtttccg-5′ |
X, 8-oxoG; Y, DHT, 8-oxoG, uracil, AP, reduced AP site, β-SSB, β–δ-SSB, HAP1-SSB or the normal corresponding base (T for DHT, uracil, AP and relatives, G for 8-oxoG); N, normal base complementary to the Y base (A opposite uracil, AP or DHT, C opposite 8-oxoG). –5 to –1, position on the complementary strand of the X base 3′ from the Y base. +1 to +5, position on the complementary strand of the X base 5′ from the Y base.