Table 3.
Characterization of the TRX homologs used in this report.
| Source | Tm1 | Cpmax2 | Hyst.3 | Gbar4 | θnat5 | θTS | θ unf | θRP |
|---|---|---|---|---|---|---|---|---|
| E. coli | 359 | 13.9 | 10.0 | 0.6 | 0.9 | 1.8 | 2.2 | 1.2 |
| S. aureus | 343 | 11.3 | 9.9 | 1.2 | 1.2 | 1.6 | 2.1 | 1.2 |
| Spinach-m cholorplast | 349 | 11.1 | 18.8 | 1.0 | 1.3 | 1.7 | 2.2 | 1.3 |
| Anabaena sp. | 366 | 25.3 | 62.8 | 5.4 | 1.0 | 1.6 | 2.4 | 1.4 |
| Human mitochondria | 376 | 21.3 | 61.0 | 2.5 | 1.1 | 1.7 | 2.2 | 1.3 |
| C. reinhardtii cytosol | 385 | 20.4 | 67.9 | 5.1 | 1.1 | 1.7 | 2.3 | 1.3 |
| Spinach-f cholorplast | 366 | 7.9 | 9.0 | 0.4 | 1.5 | 1.8 | 2.3 | 1.4 |
| Fruit fly cytosol | 379 | 15.6 | 30.0 | 1.9 | 1.3 | 1.9 | 2.5 | 1.4 |
| Human cytosol | 369 | 19.9 | 52.9 | 3.6 | 1.2 | 1.7 | 2.4 | 1.3 |
| Average | 365.8 | 16.3 | 35.8 | 2.4 | 1.2 | 1.7 | 2.3 | 1.3 |
| Standard deviation | 13.7 | 5.8 | 25.2 | 1.9 | 0.2 | 0.1 | 0.1 | 0.1 |
1
Melting temperature (K).
2
Peak height of the heat capacity curve in units of kcal/mol·K.
3
Hysteresis temperature range (K).
4
The height of the straddling free energy barrier (units are kcal/mol) separating the native and unfolded basins within the one-dimensional free energy landscapes.
5
Key points along the one-dimensional free energy landscape at the respective Tm are provided: θnat ≡ minimum of the native free energy basin, θTS ≡ location of the transition state barrier, θunf ≡ the minimum of the unfolded free energy basin, and θRP ≡ rigid cluster percolation threshold.