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. Author manuscript; available in PMC: 2011 Nov 19.
Published in final edited form as: J Mol Biol. 2010 Sep 8;404(1):45–55. doi: 10.1016/j.jmb.2010.08.054

Figure 4.

Figure 4

Representative curves demonstrating that C-terminal ASIP, AgRP and various chimera proteins inhibit NDP-MSH stimulated cAMP production at MC1R and MC4R. (Note: curves not shown here are available in the Supplement.) Experiments were performed on transfected HEK 293 cells pretreated with the ligand of interest. A rightward shift relative to NDP-MSH alone (no antagonist) reflects antagonist function. EC50 values are reported in Table 3. At MC1R, several chimeras stimulated cAMP release, thus functioning as agonists, and are not shown.