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. 2001 Mar 1;29(5):1222–1227. doi: 10.1093/nar/29.5.1222

Figure 2.

Figure 2

(A) Northern blot analysis of p53 transcripts in the yeast strains yp53 (P) and yΔp53 (Δ). The lower part represents the ethidium bromide-stained gel and the upper part shows the autoradiograph using p53 cDNA as a probe. (B) Western blot analysis of p53 protein expression in the two recombinant yeast strains yp53 (P) and yΔp53 (Δ) using the HR53C1 p53 monoclonal antibody. (C) Kinetics of p53 expression. The yeast strains yp53 (P) and yΔp53 (Δ) were cultivated at 28°C in rich medium with 0.5% glucose, until OD = 3, then 2% galactose was added and aliquots taken at 6, 12 and 24 h. p53 was immunoblotted for the monoclonal antibody HR53C1. Note the cross-reaction with two yeast proteins (y1 and y2), which serve as internal control of loading.