Figure 4.

(A) Schematic representation of p53 constructs used in this study. The p53 ORF (open), 5′-UTR (black) and 3′-UTR (grey) are represented. (B) Western blot of p53 protein expression in the recombinant yeast strains yp53 (P), yΔp53 (Δ), y5′Δp53 (5′Δ) and y3′Δp53 (3′Δ). The cultures were stopped at 6 h galactose post-induction. HR53C1 was used as p53 primary monoclonal antibody.