Fig. 2.

The effects of tunicamycin and thapsigargin on TRB3 protein expression in INS-1 cells. Western blot analysis of TRB3 protein expression in the presence of tunicamycin (A) and thapsigargin (B). INS-1 cells were incubated with tunicamycin (2 µg/mL) and thapsigargin (1 µM) for indicated times. β-actin protein levels were analyzed as an internal control. The data in bar graph are the mean ± SEM of three independent measurements. ^aP < 0.01 and ^bP < 0.001 compared to 0 hour.