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. 2001 Mar 1;29(5):1080–1086. doi: 10.1093/nar/29.5.1080

Figure 2.

Figure 2

TED crosslinks to at least seven wheat germ proteins. Radiolabelled TED(655–753) RNA (5 fmol/µl) was incubated with or without wheat germ extract (WG). The RNA–protein interactions were crosslinked with UV light (Xlink), except for the no-crosslinking control. The unbound RNA was degraded with RNase A and RNase VI in the absence or presence of proteinase K (PK). The samples were separated on an SDS–polyacrylamide gel. The molecular weight of the marker proteins (M) in kDa is indicated on the right, and the calculated molecular weight of the TED-binding proteins is indicated on the left.