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Kinetic parameters for CDNB metabolism.
| Isoform | Treatment | KM (mM) | Vmax (μmol min−1 mg−1) | kcat (s−1) | Catalytic efficiency (kcat/KM, s−1 mM−1) |
|---|---|---|---|---|---|
| GST A1-1 | Control | 0.31 ± 0.06 | 41 ± 3 | 35 ± 2 | 113 ± 24 |
| +HNE | 0.5 ± 0.2 | 31 ± 4 | 27 ± 4 | 58 ± 23 | |
| GST A4-4 | Control | 1.2 ± 0.1 | 6.3 ± 0.4 | 5.4 ± 0.4 | 4.4 ± 0.6 |
| +HNE | 1.4 ± 0.5 | 6 ± 1 | 5 ± 1 | 3.5 ± 1.4 | |
| GST P1-1 | Control | 1.7 ± 0.2 | 95 ± 8 | 74 ± 6 | 43 ± 7 |
| +HNE | 1.0 ± 0.2 | 30 ± 3 | 23 ± 2 | 24 ± 5 |
