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. 2010 Jul 14;217(3):203–213. doi: 10.1111/j.1469-7580.2010.01269.x

Table 1.

RT-PCR primer sequences.

Target gene/ref Forward primer (5′-3′) Reverse primer (5′-3′)
Oct4 primary PCR TAGGTGAGCCGTCTTTCCAC CTCGAACCACATCCTTCTCT
Oct4 secondary PCR GTGAGCCGTCTTTCCACCAGG TGATTGGCGATGTGAGTGAT
Oct4 QPCR GTGAGCCGTCTTTCCACCAGG GGGTGAGAAGGCGAAGTCTG
Nanog (Robertson et al., 2006) ATGAAGTGCAAGCGGTGGCAGAAA CCTGGTGGAGTCACAGAGTAGTTC
Sox2 (Takahashi & Yamanaka, 2006) AAGTACACGCTTCCCGGAGGCTTG AGTGGGAGGAAGAGGTAACCAC
c-Myc (Takahashi & Yamanaka, 2006) GAGTGCATTGACCCCTCAGT AATTCAGGGATCTGGTCACG
Gapdh GGGTGGAGCCAAACGGGTC GGAGTTGCTGTTGAAGTCGCA
Beta Actin GTATGCCTCGGTCGTACCA CTTCTGCATCCTGTCAGCAA
Clathrin GACAGTGCCATCATGAATCC TTTGTGCTTCTGGAGGAAAGAA