Table 1.
Target gene/ref | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|
Oct4 primary PCR | TAGGTGAGCCGTCTTTCCAC | CTCGAACCACATCCTTCTCT |
Oct4 secondary PCR | GTGAGCCGTCTTTCCACCAGG | TGATTGGCGATGTGAGTGAT |
Oct4 QPCR | GTGAGCCGTCTTTCCACCAGG | GGGTGAGAAGGCGAAGTCTG |
Nanog (Robertson et al., 2006) | ATGAAGTGCAAGCGGTGGCAGAAA | CCTGGTGGAGTCACAGAGTAGTTC |
Sox2 (Takahashi & Yamanaka, 2006) | AAGTACACGCTTCCCGGAGGCTTG | AGTGGGAGGAAGAGGTAACCAC |
c-Myc (Takahashi & Yamanaka, 2006) | GAGTGCATTGACCCCTCAGT | AATTCAGGGATCTGGTCACG |
Gapdh | GGGTGGAGCCAAACGGGTC | GGAGTTGCTGTTGAAGTCGCA |
Beta Actin | GTATGCCTCGGTCGTACCA | CTTCTGCATCCTGTCAGCAA |
Clathrin | GACAGTGCCATCATGAATCC | TTTGTGCTTCTGGAGGAAAGAA |