Fig. 4.

Expression of Ten-m/Odz3 in ATDC5 cells. To assess the expression pattern of Ten-m/Odz3 during chondrogenic differentiation and maturation in vitro, total RNA was isolated from ATDC5 cells on days 5, 8, 11, 14, 17, 20, 23, and 26 during the culture period and subjected to reverse transcription. The expression levels of Ten-m/Odz3 (A), type I collagen (B), type II collagen (C), and type X collagen (D) were analyzed by reverse transcription-polymerase chain reaction. Hypoxanthine phosphoribosyltransferase (E) was amplified as a positive control. Ten-m/Odz3 was strongly expressed during the early stage of chondrogenic differentiation in the ATDC5 cell culture.