Figure 2.

Metatarsal Differentiation. Morphology of metatarsals cultured with 1.25 mM and 7 mM phosphate and stained with H&E (A). TB, 0.1% Toluidine blue O stain; VK (Von Kossa) stain and in situ hybridization analysis for Col II, Col X, OP were performed on sections of metatarsals cultured with 1.25 and 7 mM phosphate for 4 days (B), 8 days (C) and 12 days (D). Data are representative of investigations performed on at least 2 sections of each of three metatarsals for each condition.