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. Author manuscript; available in PMC: 2010 Nov 4.
Published in final edited form as: J Cell Biochem. 2009 Oct 15;108(3):668–674. doi: 10.1002/jcb.22302

Figure 3.

Figure 3

Phosphate increases FGF18 expression. FGF18 (A) and BMP2 (B) expression were determined by quantitative real time PCR of RNA isolated from metatarsals cultured with 7mM and 1.25 mM phosphate for 24 hours in culture. Expression levels were normalized to β-actin in each sample. Error bars represent mean ± SEM. *P<0.05. STAT1 immunoreactivity is observed in vivo in hypertrophic, but not proliferative chondrocytes, in the humerus of e14.5 mice. Asterisks point to blood vessels. Arrows point to STAT positive hypertrophic chondrocytes in the humerus. No immunoreactivity is observed in the hypertrophic chondrocytes of metatarsals of e14.5 mice. Data are representative of that obtained from at least 3 independent experiments.