Figure 1.

Three-state thermal unfolding of WT α-synuclein, the PD-linked A53T and E46K mutants, and a C-terminal-truncation variant (residues 1–107). Protein-denaturation transitions were monitored by far-UV CD spectroscopy in the presence of SDS (1 mm). Unfolding data were collected from 0 to 100°C for all peptides except αSyn 1–107 (0–70°C), which aggregates at higher temperatures under the experimental conditions used (see the Supporting Information for additional details).