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. Author manuscript; available in PMC: 2010 Nov 4.
Published in final edited form as: Chem Biol Interact. 2007 Nov 19;172(1):72–80. doi: 10.1016/j.cbi.2007.11.002

Figure 3.

Figure 3

Activation of AP-1 by BPDE and the effect of cadmium on BPDE-induced AP-1 activation. Determination of AP-1 activation corresponding to luciferase activity in the extracts of Cl41 cells harboring AP-1 -responsive luciferase reporter gene has been described elsewhere. (A) Cells were harvested at different time points after BPDE (1 μM) treatment, (B) cells were treated with different concentrations of BPDE for 90 minutes in serum-free medium followed by harvesting 16 hours after BPDE treatment and (C) cells were either untreated or treated with BPDE alone, CdCl2 alone or pre-treated with CdCl2 for 4 hours followed by BPDE (1 μM) for 90 minutes. After treatments cells were incubated in 5% serum-containing MEM and harvested 16 hours after BPDE treatment. The results are presented as relative AP-1 activation (fold increase) compared to untreated cells. Each bar indicates the mean ± SD of three parallel experiments.