Fig. 1.
UV and fluorescence spectral profiles of hIgG (5mg/ml) with glyoxal (5, 20 and 40 mM) at 37°C for 30 days. UV spectral profiles (a) measured at 280 nm. Fluorescence spectral profiles (b) measured at an excitation wavelength of 340 nm and an emission wavelength of 420 nm. All points in graphs (a) and (b) represent the average of duplicate measurements, and every reading was found to be within 5% of its counterpart duplicate. All readings were compared to the highest reading acquired with methylglyoxal set at 100%.