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. Author manuscript; available in PMC: 2011 Oct 19.
Published in final edited form as: Biochemistry. 2010 Oct 19;49(41):8818–8830. doi: 10.1021/bi100557v

Figure 1.

Figure 1

Fluorescence polarization anisotropy (FPA) measurement (circles) of SgrAI binding to FLO labeled PCP in Buffer 3 (Table 1 legend) at 4°C. The line shows a fit (see Methods) to a 1:1 binding model giving a KD of 10.8±0.5 nM with R=0.99965.