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. Author manuscript; available in PMC: 2011 Oct 19.

Published in final edited form as: Biochemistry. 2010 Oct 19;49(41):8818–8830. doi: 10.1021/bi100557v

Table 2.

Single turnover DNA cleavage rate constants using 1 µM SgrAI and 37°C (unless otherwise noted)

³²P labeled DNA (1 nM)	Conc. Added unlabeled DNA	WT SgrAI Rate constant (min⁻¹)
1° site (18-1, 18 bp)	0	0.094±0.015
	10 nM PCP	0.18±0.06
	100 nM PCP	0.30±0.03
	1 µM PCP	>20
	1 µM PCP (QF)	22±7
	2 µM PCP	>20
	0.9 µM 18-1	0.063±0.006
	0.9 µM 40-1	10.0±1.4 (56±2%) 0.032±0.012 (44±2%)
	0.9 µM 40-2	0.16±0.06
	0, 4°C	<4×10−^5b
	0.9 µM 18-1, 4°C	0.118±0.014
	0.9 µM PC, 4°C	2.2±0.04
1° site (40-1, 40 bp)	0	0.14±0.05
	0.9 µM 40-1	>20 (22±9%) 0.024±0.008 (78±9%)
2° site (18-2, 18 bp)	0	0.020±0.006 (2.8%±0.6%)
	10 nM PCP	0.012±0.001^a (3.4%±0.1%) ^a
	100 nM PCP	0.016±0.005 (19%±5%)
	1 µM PCP	0.05±0.01 (24%±1%)

^a

Only 2 repetitions.

QF=measurement made in the Kintek RQF-3 Rapid Quench Flow Instrument.

^b

No cutting detected after 21 hours of incubation. Rate constant estimated assuming 5% cleavage or less after 21 hours.