Figure 5.

CU-NP abrogates calcineurin activation. (A–C) The effect of CU-NP on calcineurin activity in myocytes at various time points after agonist addition as determined by phosphatase activity assay, whereas (D) to (F) show gene expression levels of MCIP 24 h after agonist administration. (G) NFAT immunofluorescence staining for NFAT, whereas (H) shows the ratio of the fluorescence intensity of the nuclear region to that of the entire region of the cell on a confocal plane. As results were identical, data were pooled for all agonists for fluorescence data. Values indicate fold increase and are given as means ± SEM. n = 6. *P < 0.05 vs. control, ^#P < 0.05 vs. hypertrophic stimuli. Ctl, control; PE, phenylephrine; Ang II, angiotensin II; ET-1, endothelin-1; Ag, agonists.