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. 2010 Sep 9;19(23):4573–4586. doi: 10.1093/hmg/ddq385

Figure 5.

Figure 5.

PSA inhibition enhances aggregate formation in cells expressing other aggregate-prone proteins (ataxin-3, mutant SOD1, mutant α-synuclein). (A and B) PSA inhibition increased aggregate formation in 293A cells expressing full-length ataxin-3 GFP with 28Q (A) and 84Q (B). The proportion of transfected cells with macroaggregates was also increased by inhibition of PSA in cells transfected with mutant SOD1 GFP G37R (C) or G93A (D). ***P < 0.001, **P < 0.01, *P < 0.05 by t-test. (E and F) Clearance of mutant α-synuclein (A53T) was slowed by inhibition of PSA. Stable inducible PC12 cells were treated with doxycycline to induce expression of mutant α-synuclein, and subsequently expression was turned off by removal of doxycycline from the medium. When PSA inhibitors were added after transgene expression was switched off, more α-synuclein remained present, indicating a decreased clearance. Levels of α-synuclein remaining after 16 h were assayed by western blot, a representative image is shown in (E). Densitometric quantification of this triplicate experiment is shown in (F). **P < 0.01, *P < 0.05, by ANOVA.