Figure 4. Testing the effect of restricted running wheel on anticipatory activity.

(A) Mice have access to a “low profile” running wheel or a (B) a shelter dome or have their bedding slightly disturbed as another control group (not shown). (C) The total number of seconds of high activity during the four hours prior to receiving running wheel access. (D) The fraction of frames during the entire twenty-two hours of recording during which the mice exhibited the high activity behaviors. (E) Normalized anticipatory activity. The number of seconds during which the mice exhibited high activity behaviors in the four hours preceding wheel or dome access or bedding disturbance is divided by the total number of seconds of high activity observed in the entire twenty-two hour recording period. (F) Food bin entry during the four hours preceding running wheel access. (G) The amount of time the mouse was awake during running wheel access, dome access, or after bedding disturbance. For wheel access mice the sum of the time it spent running on the wheel, interacting with the dome or wheel, or awake but not interaction is equivalent to time awake (Kruskal-Wallis Test, * = p<0.05, ** = p<0.01) * = p<0.05, Kruskal-Wallis Test.