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. 2010 Nov 3;5(11):e15433. doi: 10.1371/journal.pone.0015433

Figure 1. Cardiac GPER expression increases in salt-fed mRen2.Lewis females.

Figure 1

a. Cardiac GPER gene expression in female mRen2.Lewis rats fed a normal salt (NS) or high salt (HS) diet determined by real-time PCR and expressed as the ratio of GPER mRNA to S16 ribosomal RNA. Values are mean ± SEM; ** P<0.01. b. Left, representative GPER immunostaining in a cross section of the heart from an intact mRen2.Lewis female. Right, representative control section stained with pre-adsorbed primary antibody. c. Full-length immunoblot of cardiac membranes showing the specificity of the primary antibody using for immunohistochemistry as evidenced by a single band corresponding to the appropriate molecular weight of GPER (∼50 kDa).