Table 2. Comparison of inhibition of rabbit β-globin synthesis in cell-free translation systems and hybridization potential predicted by probability profile for rabbit β-globin mRNA.
| ASO name | Target sequence/site | % Inhibitiona | Hybridization | Reference |
| (length in nt) |
on mRNA |
|
potential |
|
| β1 (20) | A14–C33/5′-UTR | 23 (5.2) | high | 45 |
| β2 (20) | C46–G65/start | 61 (5.2) | high | 45 |
| β3 (20) | A144–C163/coding | 18 (5.2) | moderate | 45 |
| β4 (20) | G207–A226/coding | 43 (5.2) | high | 45 |
| β5 (22) | A1–G22/cap | 67 (5.2) | high | 45 |
| β6 (23) | U23–A45/5′-UTR | 47 (5.2) | high | 45 |
| β7 (CCC+β5, 25) | A1–G22/cap | 75 (5.2) | high | 45 |
| β8 (β7β6, 48) | A1–A45/cap | 89 (2.6) | high | 45 |
| β6+β7 (mixture) | A1–A45/cap | 89 (2.6) | high | 45 |
| BG1 (17) | C46–U62/start | 50 (0.1) | high | 24 |
| BG2 (17) | A51–C67/start | 50 (0.5) | high | 24 |
| BG3 (15) | C85–U95/coding | 0 (1) | low | 24 |
| 17 Glo[3-19] (17) | A3–A19/cap | 72 (0.5) | high | 44 |
| 17 Glo[51–67] (17) | U51–C67/start | 95 (0.5) | high | 44 |
| 11 Glo (11) | A44–A54/start | 65 (0.5) | high | 44 |
| 17 Glo[113–129] (17) | U113–G129 / coding | 95 (0.5) | low | 44 |
aNumbers in parentheses represent ASO concentration in µM.